HCK monoclonal antibody (M01), clone 1D9-1A9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of HCK expression in transfected 293T cell line by HCK monoclonal antibody (M01), clone 1D9-1A9.
Lane 1: HCK transfected lysate (Predicted MW: 57.3 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of HCK transfected lysate using anti-HCK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HCK MaxPab rabbit polyclonal antibody.
Western Blot detection against Immunogen (81.29 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant HCK.
Immunogen
HCK (AAH14435, 1 a.a. ~ 505 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MGCMKSKFLQVGGNTSSKTETSASPHCPVYVPDPTSTIKPGPNSHNSNTPGIREAGSEDIIVVALYDYEAIHHEDLSFQKGDQMVVLEESGEWWKARSLATRKEGYIPSNYVARVDSLETEEWFFKGISRKDAERQLLAPGNMLGSFMIRDSETTKGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRSTFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGVSNPEVIRALERGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (81.29 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of HCK expression in transfected 293T cell line by HCK monoclonal antibody (M01), clone 1D9-1A9.
Lane 1: HCK transfected lysate (Predicted MW: 57.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of HCK transfected lysate using anti-HCK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HCK MaxPab rabbit polyclonal antibody.ELISA
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Gene Info — HCK
Entrez GeneID
3055GeneBank Accession#
BC014435Protein Accession#
AAH14435Gene Name
HCK
Gene Alias
JTK9
Gene Description
hemopoietic cell kinase
Omim ID
142370Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization. [provided by RefSeq
Other Designations
tyrosine protein kinase HCK
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Interactome
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Pathway
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Disease
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