HARS 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-HARS full-length
Host
Human
Theoretical MW (kDa)
56.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-HARS antibody (H00003035-B01) by Western Blots.
Western Blot
Lane 1: HARS transfected lysate ( 57.4 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
HARS transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — HARS
Entrez GeneID
3035GeneBank Accession#
NM_002109Protein Accession#
NP_002100Gene Name
HARS
Gene Alias
FLJ20491, HRS
Gene Description
histidyl-tRNA synthetase
Omim ID
142810Gene Ontology
HyperlinkGene Summary
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. [provided by RefSeq
Other Designations
HisRS|histidine tRNA ligase 1, cytoplasmic|histidine translase|histidine-tRNA ligase
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