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HARS 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00003035-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-HARS full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 56.1
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-HARS antibody (H00003035-B01) by Western Blots.
    Western Blot
    QC Testing of H00003035-T01
    Lane 1: HARS transfected lysate ( 57.4 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00003035-T01
    HARS transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 3035
  • Gene Name:
  • HARS
  • Gene Alias:
  • FLJ20491,HRS
  • Gene Description:
  • histidyl-tRNA synthetase
  • Gene Summary:
  • Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. [provided by RefSeq
  • Other Designations:
  • HisRS,histidine tRNA ligase 1, cytoplasmic,histidine translase,histidine-tRNA ligase
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