HARS monoclonal antibody (M03A), clone 4D4
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More Files
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Western Blot (Cell lysate)
HARS monoclonal antibody (M03A), clone 4D4. Western Blot analysis of HARS expression in HeLa ( Cat # L013V1 ).
Western Blot detection against Immunogen (36.3 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant HARS.
Immunogen
HARS (NP_002100, 1 a.a. ~ 96 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPV
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.3 KDa) .
Storage Buffer
In ascites fluid
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
HARS monoclonal antibody (M03A), clone 4D4. Western Blot analysis of HARS expression in HeLa ( Cat # L013V1 ).Western Blot (Recombinant protein)
ELISA
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Gene Info — HARS
Entrez GeneID
3035GeneBank Accession#
NM_002109Protein Accession#
NP_002100Gene Name
HARS
Gene Alias
FLJ20491, HRS
Gene Description
histidyl-tRNA synthetase
Omim ID
142810Gene Ontology
HyperlinkGene Summary
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. [provided by RefSeq
Other Designations
HisRS|histidine tRNA ligase 1, cytoplasmic|histidine translase|histidine-tRNA ligase
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Interactome
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Pathway
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