H3F3B rabbit monoclonal antibody
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Specification
Product Description
Rabbit monoclonal antibody raised against a human H3F3B peptide using ARM Technology.
Immunogen
A synthetic peptide of human H3F3B is used for rabbit immunization.
Customer or Abnova will decide on the preferred peptide sequence.Host
Rabbit
Library Construction
Non-fusion antibody library from rabbit spleen (ARM Technology).
Expression
Overexpression vector and transfection into 293H cell line.
Reactivity
Human
Purification
Protein A
Isotype
IgG
Quality Control Testing
Antibody reactive against human H3F3B peptide by ELISA and mammalian transfected lysate by Western Blot.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Deliverable
Up to three rabbit IgG clones of 100 ug each will be delivered to customer.
Note
1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request. -
Applications
Western Blot (Transfected lysate)
ELISA
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Gene Info — H3F3B
Entrez GeneID
3021GeneBank Accession#
H3F3BGene Name
H3F3B
Gene Alias
H3.3B, H3F3A
Gene Description
H3 histone, family 3B (H3.3B)
Omim ID
601058Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the histone H3 family. [provided by RefSeq
Other Designations
H3 histone, family 3A|H3 histone, family 3B
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Interactome
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Pathway
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Publication Reference
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Dynamics of Asymmetric and Symmetric Divisions of Muscle Stem Cells In Vivo and on Artificial Niches.
Evano B, Khalilian S, Le Carrou G, Almouzni G, Tajbakhsh S.
Cell Reports 2020 Mar; 30(10):3195.
Application：WB-Ce, Mouse, primary embryonic fibroblasts.
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Dynamics of Asymmetric and Symmetric Divisions of Muscle Stem Cells In Vivo and on Artificial Niches.
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