H2AFX 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
H2AFX transfected lysate.
Western Blot
Lane 1: H2AFX transfected lysate ( 15.1 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-H2AFX full-length
Host
Human
Theoretical MW (kDa)
15.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-H2AFX antibody (H00003014-D01P) by Western Blots.
SDS-PAGE Gel
H2AFX transfected lysate.
Western Blot
Lane 1: H2AFX transfected lysate ( 15.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — H2AFX
Entrez GeneID
3014GeneBank Accession#
NM_002105.2Protein Accession#
NP_002096.1Gene Name
H2AFX
Gene Alias
H2A.X, H2A/X, H2AX
Gene Description
H2A histone family, member X
Omim ID
601772Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq
Other Designations
H2AX histone
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Interactome
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Pathway
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Disease
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