GUSB 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GUSB full-length
Host
Human
Theoretical MW (kDa)
71.72
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GUSB antibody (H00002990-B01) by Western Blots.
SDS-PAGE Gel
GUSB transfected lysate.
Western Blot
Lane 1: GUSB transfected lysate ( 71.72 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GUSB
Entrez GeneID
2990GeneBank Accession#
NM_000181.1Protein Accession#
NP_000172.1Gene Name
GUSB
Gene Alias
FLJ39445, MPS7
Gene Description
glucuronidase, beta
Gene Ontology
HyperlinkGene Summary
The GUSB gene encodes beta-glucuronidase (EC 3.2.1.31), a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al., 1993 [PubMed 7680524]). It is a tetrameric glycoprotein composed of identical subunits (Oshima et al., 1987 [PubMed 3468507]). The GUSB gene is mutated in mucopolysaccharidosis type VII (MPS7; MIM 253220).[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Disease
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