GUCA1A monoclonal antibody (M04), clone 2F7
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant GUCA1A.
Immunogen
GUCA1A (NP_000400, 1 a.a. ~ 93 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MGNVMEGKSVEELSSTECHQWYKKFMTECPSGQLTLYEFRQFFGLKNLSPSASQYVEQMFETFDFNKDGYIDFMEYVAALSLVLKGKVEQKLR
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.97 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of GUCA1A expression in transfected 293T cell line by GUCA1A monoclonal antibody (M04), clone 2F7.
Lane 1: GUCA1A transfected lysate(22.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of GUCA1A over-expressed 293 cell line, cotransfected with GUCA1A Validated Chimera RNAi ( Cat # H00002978-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GUCA1A monoclonal antibody (M04), clone 2F7 (Cat # H00002978-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — GUCA1A
Entrez GeneID
2978GeneBank Accession#
NM_000409Protein Accession#
NP_000400Gene Name
GUCA1A
Gene Alias
COD3, GCAP, GCAP1, GUCA, GUCA1
Gene Description
guanylate cyclase activator 1A (retina)
Gene Ontology
HyperlinkGene Summary
This gene plays a role in the recovery of retinal photoreceptors from photobleaching. In the recovery phase, the phototransduction messeneger cGMP is replenished by retinal guanylyl cyclase-1 (GC1). GC1 is activated by decreasing Ca(2+) concentrations following photobleaching. The protein encoded by this gene, guanylyl cyclase activating protein 1 (GCAP1), mediates the sensitivity of GC1 to Ca(2+) concentrations. GCAP1 promotes activity of GC1 at low Ca(2+) concentrations and inhibits GC1 activity at high Ca(2+) concentrations. Mutations in this gene cause autosomal dominant cone dystrophy (COD3); a disease characterized by reduced visual acuity associated with progressive loss of color vision. Mutations in this gene prohibit the inactivation of RetGC1 at high Ca(2+) concentrations; causing the constitutive activation of RetGC1 and, presumably, increased cell death. This gene is expressed in retina and spermatagonia. [provided by RefSeq
Other Designations
OTTHUMP00000016397|OTTHUMP00000196466
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