BRF1 293T Cell Transient Overexpression Lysate(Denatured)

Catalog # H00002972-T01

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Size:100 uL
Price: USD $ 247.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
SDS-PAGE Gel
QC Test

SDS-PAGE Gel

BRF1 transfected lysate.

Western Blot
QC Test

Western Blot

Lane 1: BRF1 transfected lysate ( 51.8 KDa)
Lane 2: Non-transfected lysate.

  • Specification

    Transfected Cell Line

    293T

    Plasmid

    pCMV-BRF1 full-length

    Host

    Human

    Theoretical MW (kDa)

    51.8

    Quality Control Testing

    Transient overexpression cell lysate was tested with Anti-BRF1 antibody (H00002972-B01) by Western Blots.

    SDS-PAGE Gel

    BRF1 transfected lysate.

    Western Blot

    Lane 1: BRF1 transfected lysate ( 51.8 KDa)
    Lane 2: Non-transfected lysate.

    Storage Buffer

    1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

    Storage Instruction

    Store at -80°C. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

  • Gene Info — BRF1

    Entrez GeneID

    2972

    GeneBank Accession#

    NM_145685.1

    Protein Accession#

    -

    Gene Name

    BRF1

    Gene Alias

    BRF, FLJ42674, FLJ43034, GTF3B, MGC105048, TAF3B2, TAF3C, TAFIII90, TF3B90, TFIIIB90, hBRF

    Gene Description

    BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae)

    Omim ID

    604902

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Two alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. Other transcript variants are possible, but their full-length natures have not been completely characterized. [provided by RefSeq

    Other Designations

    B - related factor 1|TATA box binding protein (TBP)-associated factor 3C|TATA box binding protein (TBP)-associated factor, RNA polymerase III, GTF3B subunit 2|TATA box binding protein (TBP)-associated factor, RNA polymerase III, subunit 2|TBP - associated

  • Interactome
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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