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GTF2H2 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00002966-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-GTF2H2 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 44.4
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-GTF2H2antibody (H00002966-D01P)byWesternBlots.
    SDS-PAGE Gel
    QC Testing of H00002966-T02
    GTF2H2 transfected lysate.
    Western Blot
    QC Testing of H00002966-T02
    Lane 1: GTF2H2 transfected lysate ( 44.4 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 2966
  • Gene Name:
  • GTF2H2
  • Gene Alias:
  • BTF2,BTF2P44,MGC102806,T-BTF2P44,TFIIH
  • Gene Description:
  • general transcription factor IIH, polypeptide 2, 44kDa
  • Gene Summary:
  • This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is within the telomeric copy of the duplication. Deletion of this gene sometimes accompanies deletion of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients but it is unclear if deletion of this gene contributes to the SMA phenotype. This gene encodes the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH which is involved in basal transcription and nucleotide excision repair. Transcript variants for this gene have been described, but their full length nature has not been determined. A second copy of this gene within the centromeric copy of the duplication has been described in the literature. It is reported to be different by either two or four base pairs; however, no sequence data is currently available for the centromeric copy of the gene. [provided by RefSeq
  • Other Designations:
  • general transcription factor IIH, polypeptide 2 (44kD subunit),general transcription factor IIH, polypeptide 2, 44kD subunit
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