GTF2A2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GTF2A2 transfected lysate.
Western Blot
Lane 1: GTF2A2 transfected lysate ( 12.10 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GTF2A2 full-length
Host
Human
Theoretical MW (kDa)
12.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GTF2A2 antibody (H00002958-B01) by Western Blots.
SDS-PAGE Gel
GTF2A2 transfected lysate.
Western Blot
Lane 1: GTF2A2 transfected lysate ( 12.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GTF2A2
Entrez GeneID
2958GeneBank Accession#
BC000287.2Protein Accession#
AAH00287.1Gene Name
GTF2A2
Gene Alias
HsT18745, TF2A2, TFIIA
Gene Description
general transcription factor IIA, 2, 12kDa
Omim ID
600519Gene Ontology
HyperlinkGene Summary
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).[supplied by OMIM
Other Designations
OTTHUMP00000196512|OTTHUMP00000196515|general transcription factor IIA, 2 (12kD subunit)
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Interactome
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Pathway
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