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GTF2A2 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00002958-T02
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-GTF2A2 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 12.1
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-GTF2A2 antibody (H00002958-B02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00002958-T02
    GTF2A2 transfected lysate.
    Western Blot
    QC Testing of H00002958-T02
    Lane 1: GTF2A2 transfected lysate ( 12.1 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 2958
  • Gene Name:
  • GTF2A2
  • Gene Alias:
  • HsT18745,TF2A2,TFIIA
  • Gene Description:
  • general transcription factor IIA, 2, 12kDa
  • Gene Summary:
  • Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995 [PubMed 7724559]).[supplied by OMIM
  • Other Designations:
  • OTTHUMP00000196512,OTTHUMP00000196515,general transcription factor IIA, 2 (12kD subunit)
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