PDIA3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PDIA3 transfected lysate.
Western Blot
Lane 1: PDIA3 transfected lysate ( 56.8 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PDIA3 full-length
Host
Human
Theoretical MW (kDa)
56.8
Interspecies Antigen Sequence
Mouse (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PDIA3 antibody (H00002923-B01) by Western Blots.
SDS-PAGE Gel
PDIA3 transfected lysate.
Western Blot
Lane 1: PDIA3 transfected lysate ( 56.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PDIA3
Entrez GeneID
2923GeneBank Accession#
NM_005313.4Protein Accession#
-Gene Name
PDIA3
Gene Alias
ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC
Gene Description
protein disulfide isomerase family A, member 3
Omim ID
602046Gene Ontology
HyperlinkGene Summary
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq
Other Designations
58 kDa microsomal protein|OTTHUMP00000041709|endoplasmic reticulum P58|glucose regulated protein, 58kDa|phospholipase C-alpha|protein disulfide isomerase-associated 3|protein disulfide-isomerase A3
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Interactome
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Pathway
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Disease
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