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Last updated: 2017/4/23
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RAPGEF1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00002889-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-RAPGEF1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 120.56
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-RAPGEF1 antibody (H00002889-B01) by Western Blots.
    Western Blot
    QC Testing of H00002889-T01
    Lane 1: RAPGEF1 transfected lysate ( 122.7 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00002889-T01
    RAPGEF1 transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 2889
  • Gene Name:
  • RAPGEF1
  • Gene Alias:
  • C3G,DKFZp781P1719,GRF2
  • Gene Description:
  • Rap guanine nucleotide exchange factor (GEF) 1
  • Gene Summary:
  • This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000064558,guanine nucleotide-releasing factor 2,guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene)
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