GPT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GPT transfected lysate.
Western Blot
Lane 1: GPT transfected lysate ( 54.67 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GPT full-length
Host
Human
Theoretical MW (kDa)
54.6
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GPT antibody (H00002875-D01P) by Western Blots.
SDS-PAGE Gel
GPT transfected lysate.
Western Blot
Lane 1: GPT transfected lysate ( 54.67 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GPT
Entrez GeneID
2875GeneBank Accession#
NM_005309.1Protein Accession#
NP_005300.1Gene Name
GPT
Gene Alias
AAT1, ALT1, GPT1
Gene Description
glutamic-pyruvate transaminase (alanine aminotransferase)
Omim ID
138200Gene Ontology
HyperlinkGene Summary
Glutamate-pyruvate transaminase (EC 2.6.1.2), also known as alanine aminotransferase, catalyzes the reversible conversion of L-alanine and alpha-ketoglutarate to L-glutamate and pyruvate. It has 2 distinct molecular and genetic forms: one cytoplasmic (soluble) (GPT1) and one mitochondrial (GPT2; MIM 138210). See ALTQTL1 (MIM 612363) and ALTQTL2 (MIM 612364) for information on quantitative trait loci influencing the plasma level of alanine aminotransferase.[supplied by OMIM
Other Designations
alanine aminotransferase
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Interactome
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Pathway
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