GPI monoclonal antibody (M01), clone 8B8
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More Files
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Western Blot (Cell lysate)
GPI monoclonal antibody (M01), clone 8B8. Western Blot analysis of GPI expression in MCF-7.
Western Blot (Transfected lysate)
Western Blot analysis of GPI expression in transfected 293T cell line by GPI monoclonal antibody (M01), clone 8B8.
Lane 1: GPI transfected lysate (Predicted MW: 63.1 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GPI is 0.03 ng/ml as a capture antibody.
Western Blot detection against Immunogen (86.9 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant GPI.
Immunogen
GPI (AAH04982.1, 1 a.a. ~ 558 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDNFEQLLSGAHWMDQHFRTTPLEKNAPVLLALLGIWYINCFGCETHAMLPYDQYLHRFAAYFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMRGKSTEEARKELQAAGKSPEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGALVAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSAQVTSHDASTNGLINFIKQQREARVQ
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (86.9 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
GPI monoclonal antibody (M01), clone 8B8. Western Blot analysis of GPI expression in MCF-7.Western Blot (Transfected lysate)
Western Blot analysis of GPI expression in transfected 293T cell line by GPI monoclonal antibody (M01), clone 8B8.
Lane 1: GPI transfected lysate (Predicted MW: 63.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GPI is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — GPI
Entrez GeneID
2821GeneBank Accession#
BC004982.1Protein Accession#
AAH04982.1Gene Name
GPI
Gene Alias
AMF, GNPI, NLK, PGI, PHI, SA-36
Gene Description
glucose phosphate isomerase
Omim ID
172400Gene Ontology
HyperlinkGene Summary
This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. [provided by RefSeq
Other Designations
autocrine motility factor|glucose-6-phosphate isomerase|hexose monophosphate isomerase|hexosephosphate isomerase|neuroleukin|oxoisomerase|phosphoglucose isomerase|phosphohexomutase|phosphohexose isomerase|phosphosaccharomutase|sperm antigen-36
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Interactome
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Pathway
- Amino sugar and nucleotide sugar metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Glycolysis / Gluconeogenesis
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