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GPI monoclonal antibody (M01), clone 8B8

  • Catalog # : H00002821-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a full-length recombinant GPI.
  • Immunogen:
  • GPI (AAH04982.1, 1 a.a. ~ 558 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2a Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00002821-M01
    Western Blot detection against Immunogen (86.9 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • GPI monoclonal antibody (M01), clone 8B8. Western Blot analysis of GPI expression in MCF-7.
  • PDF DownloadProtocol Download
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of GPI expression in transfected 293T cell line by GPI monoclonal antibody (M01), clone 8B8.

    Lane 1: GPI transfected lysate (Predicted MW: 63.1 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged GPI is 0.03 ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2821
  • Gene Name:
  • GPI
  • Gene Alias:
  • Gene Description:
  • glucose phosphate isomerase
  • Gene Summary:
  • This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. [provided by RefSeq
  • Other Designations:
  • autocrine motility factor,glucose-6-phosphate isomerase,hexose monophosphate isomerase,hexosephosphate isomerase,neuroleukin,oxoisomerase,phosphoglucose isomerase,phosphohexomutase,phosphohexose isomerase,phosphosaccharomutase,sperm antigen-36
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