GPD2 monoclonal antibody (M02), clone 2C11-1F4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of GPD2 expression in transfected 293T cell line by GPD2 monoclonal antibody (M02), clone 2C11-1F4.
Lane 1: GPD2 transfected lysate(41 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GPD2 is approximately 1ng/ml as a capture antibody.
Western Blot detection against Immunogen (67.32 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant GPD2.
Immunogen
GPD2 (AAH19874, 1 a.a. ~ 378 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAFQKAVKGTLVGGGALATVLGLSQFAHYRRKQMNLAYVKAADCISEPVNREPPSREAQLLTLQNTSEFDILVIGGGATGSGCALDAVTRGLKTALVERDDFSSGTSSRSTKLIHGGVRYLQKAIMKLDIEQYRMVKEALHERANLLEIAPHLSAPLPIMLPVYKWWQLPYYWVGIKLYDLVAGSNCLKSSYVLSKSRALEHFPMLQKDKLVGAIVYYDGQHNDARMNLAIALTAARYGAATANYMEVVSLLKKTDPQTGKVHVSGARCKDVLTGQEFDVRAKCVINATGPFTDSVRKMDDKDAAAICQPSAGVHIVMPGYYSPESMGLLDPATSDGRVIFFLPWQKMTIAGTTDTPTDVTHHPIPSEEDINFILNEV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (93); Rat (92)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (67.32 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of GPD2 expression in transfected 293T cell line by GPD2 monoclonal antibody (M02), clone 2C11-1F4.
Lane 1: GPD2 transfected lysate(41 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GPD2 is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — GPD2
Entrez GeneID
2820GeneBank Accession#
BC019874Protein Accession#
AAH19874Gene Name
GPD2
Gene Alias
GDH2, GPDM, mGPDH
Gene Description
glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
Gene Ontology
HyperlinkGene Summary
Mitochondrial glycerophosphate dehydrogenase (EC 1.1.99.5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.[supplied by OMIM
Other Designations
mitochondrial glycerophosphate dehydrogenase
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Interactome
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Pathway
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Disease
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