GOT2 monoclonal antibody (M09), clone 4H8
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant GOT2.
Immunogen
GOT2 (NP_002071, 331 a.a. ~ 430 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LNTPDLRKQWLQEVKGMADRIIGMRTQLVSNLKKEGSTHNWQHITDQIGMFCFTGLKPEQVERLIKEFSIYMTKDGRISVAGVTSSNVGYLAHAIHQVTK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
GOT2 monoclonal antibody (M09), clone 4H8. Western Blot analysis of GOT2 expression in HepG2(Cat # L019V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GOT2 is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — GOT2
Entrez GeneID
2806GeneBank Accession#
NM_002080Protein Accession#
NP_002071Gene Name
GOT2
Gene Alias
FLJ40994, KAT4, KATIV, mitAAT
Gene Description
glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
Omim ID
138150Gene Ontology
HyperlinkGene Summary
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq
Other Designations
aspartate aminotransferase 2|kynurenine aminotransferase IV
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Interactome
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Pathway
- Alanine
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Carbon fixation in photosynthetic organisms
- Cysteine and methionine metabolism
- Isoquinoline alkaloid biosynthesis
- Metabolic pathways
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