GOT1 (Human) IP-WB Antibody Pair
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90%); Rat (90%)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of GOT1 transfected lysate using rabbit polyclonal anti-GOT1 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-GOT1.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-GOT1 (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-GOT1 (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — GOT1
Entrez GeneID
2805Gene Name
GOT1
Gene Alias
GIG18
Gene Description
glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
Omim ID
138180Gene Ontology
HyperlinkGene Summary
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq
Other Designations
OTTHUMP00000020254|aspartate aminotransferase 1|growth-inhibiting protein 18
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Interactome
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Pathway
- Alanine
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Carbon fixation in photosynthetic organisms
- Cysteine and methionine metabolism
- Isoquinoline alkaloid biosynthesis
- Metabolic pathways
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