GNS 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GNS transfected lysate.
Western Blot
Lane 1: GNS transfected lysate ( 62.10 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GNS full-length
Host
Human
Theoretical MW (kDa)
62.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GNS antibody (H00002799-B01P) by Western Blots.
SDS-PAGE Gel
GNS transfected lysate.
Western Blot
Lane 1: GNS transfected lysate ( 62.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GNS
Entrez GeneID
2799GeneBank Accession#
NM_002076.2Protein Accession#
NP_002067.1Gene Name
GNS
Gene Alias
G6S, MGC21274
Gene Description
glucosamine (N-acetyl)-6-sulfatase
Gene Ontology
HyperlinkGene Summary
The product of this gene is a lysosomal enzyme found in all cells. It is involved in the catabolism of heparin, heparan sulphate, and keratan sulphate. Deficiency of this enzyme results in the accumulation of undegraded substrate and the lysosomal storage disorder mucopolysaccharidosis type IIID (Sanfilippo D syndrome). Mucopolysaccharidosis type IIID is the least common of the four subtypes of Sanfilippo syndrome. [provided by RefSeq
Other Designations
N-acetylglucosamine-6-sulfatase|glucosamine-6-sulfatase
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Interactome
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Pathway
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