GNGT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GNGT1 transfected lysate
Western Blot
Lane 1: GNGT1 transfected lysate ( 8.25 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GNGT1 full-length
Host
Human
Theoretical MW (kDa)
8.25
Interspecies Antigen Sequence
Mouse (97); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GNGT1 antibody (H00002792-B03) by Western Blots.
SDS-PAGE Gel
GNGT1 transfected lysate
Western Blot
Lane 1: GNGT1 transfected lysate ( 8.25 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GNGT1
Entrez GeneID
2792GeneBank Accession#
BC029367Protein Accession#
AAH29367Gene Name
GNGT1
Gene Alias
GNG1
Gene Description
guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1
Omim ID
189970Gene Ontology
HyperlinkGene Summary
Heterotrimeric guanine nucleotide-binding proteins (G proteins) transduce extracellular signals received by transmembrane receptors to effector proteins. Transducin is a guanine nucleotide-binding protein found specifically in rod outer segments, where it mediates activation by rhodopsin of a cyclic GTP-specific (guanosine monophosphate) phosphodiesterase. Transducin is also referred to as GMPase. GNGT1 encodes the gamma subunit of transducin (Hurley et al., 1984 [PubMed 6438626]; Scherer et al., 1996 [PubMed 8661128]).[supplied by OMIM
Other Designations
OTTHUMP00000024526
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Interactome
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Pathway
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