GNGT1 monoclonal antibody (M01), clone 1F8
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant GNGT1.
Immunogen
GNGT1 (AAH29367, 1 a.a. ~ 74 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPVINIEDLTEKDKLKMEVDQLKKEVTLERMLVSKCCEEVRDYVEERSGEDPLVKGIPEDKNPFKELKGGCVIS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (95)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (33.88 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of GNGT1 expression in transfected 293T cell line by GNGT1 monoclonal antibody (M01), clone 1F8.
Lane 1: GNGT1 transfected lysate(8.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GNGT1 is 1 ng/ml as a capture antibody.ELISA
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Gene Info — GNGT1
Entrez GeneID
2792GeneBank Accession#
BC029367Protein Accession#
AAH29367Gene Name
GNGT1
Gene Alias
GNG1
Gene Description
guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1
Omim ID
189970Gene Ontology
HyperlinkGene Summary
Heterotrimeric guanine nucleotide-binding proteins (G proteins) transduce extracellular signals received by transmembrane receptors to effector proteins. Transducin is a guanine nucleotide-binding protein found specifically in rod outer segments, where it mediates activation by rhodopsin of a cyclic GTP-specific (guanosine monophosphate) phosphodiesterase. Transducin is also referred to as GMPase. GNGT1 encodes the gamma subunit of transducin (Hurley et al., 1984 [PubMed 6438626]; Scherer et al., 1996 [PubMed 8661128]).[supplied by OMIM
Other Designations
OTTHUMP00000024526
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Interactome
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Pathway
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