GNAQ 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GNAQ transfected lysate
Western Blot
Lane 1: GNAQ transfected lysate ( 39.6 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GNAQ full-length
Host
Human
Theoretical MW (kDa)
39.6
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GNAQ antibody (H00002776-B01) by Western Blots.
SDS-PAGE Gel
GNAQ transfected lysate
Western Blot
Lane 1: GNAQ transfected lysate ( 39.6 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GNAQ
Entrez GeneID
2776GeneBank Accession#
BC057777Protein Accession#
AAH57777Gene Name
GNAQ
Gene Alias
G-ALPHA-q, GAQ
Gene Description
guanine nucleotide binding protein (G protein), q polypeptide
Omim ID
600998Gene Ontology
HyperlinkGene Summary
Guanine nucleotide-binding proteins are a family of heterotrimeric proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit. G-alpha-q is the alpha subunit of one of the heterotrimeric GTP-binding proteins that mediates stimulation of phospholipase C-beta (MIM 600230).[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Disease
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