GNAI2 monoclonal antibody (M02), clone 3G11
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant GNAI2.
Immunogen
GNAI2 (AAH12138, 1 a.a. ~ 355 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (98); Rat (99)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (64.57 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
GNAI2 monoclonal antibody (M02), clone 3G11. Western Blot analysis of GNAI2 expression in MCF-7 ( Cat # L046V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GNAI2 is approximately 0.3ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between C5AR1 and GNAI2. HeLa cells were stained with anti-C5AR1 rabbit purified polyclonal 1:1200 and anti-GNAI2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — GNAI2
Entrez GeneID
2771GeneBank Accession#
BC012138Protein Accession#
AAH12138Gene Name
GNAI2
Gene Alias
GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1
Gene Description
guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Gene Ontology
HyperlinkGene Summary
O
Other Designations
GTP-binding regulatory protein Gi alpha-2 chain|WUGSC:H_LUCA15.1|WUGSC:H_LUCA16.1
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Interactome
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Pathway
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Disease
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