GLUL 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GLUL transfected lysate.
Western Blot
Lane 1: GLUL transfected lysate ( 42.10 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GLUL full-length
Host
Human
Theoretical MW (kDa)
42.1
Interspecies Antigen Sequence
Mouse (95); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GLUL antibody (H00002752-D01P) by Western Blots.
SDS-PAGE Gel
GLUL transfected lysate.
Western Blot
Lane 1: GLUL transfected lysate ( 42.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GLUL
Entrez GeneID
2752GeneBank Accession#
NM_002065.4Protein Accession#
NP_002056.2Gene Name
GLUL
Gene Alias
GLNS, GS, PIG43, PIG59
Gene Description
glutamate-ammonia ligase (glutamine synthetase)
Gene Ontology
HyperlinkGene Summary
Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM
Other Designations
OTTHUMP00000035524|OTTHUMP00000035525|cell proliferation-inducing protein 59|glutamate-ammonia ligase (glutamine synthase)|glutamine synthetase|proliferation-inducing protein 43
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Interactome
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Pathway
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Disease
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