GLS monoclonal antibody (M05), clone 6E12
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant GLS.
Immunogen
GLS (NP_055720, 580 a.a. ~ 669 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
EQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGLL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (98)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.64 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GLS is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — GLS
Entrez GeneID
2744GeneBank Accession#
NM_014905Protein Accession#
NP_055720Gene Name
GLS
Gene Alias
AAD20, DKFZp686O15119, FLJ10358, GLS1, KIAA0838
Gene Description
glutaminase
Omim ID
138280Gene Ontology
HyperlinkGene Summary
Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM
Other Designations
K-glutaminase|L-glutamine amidohydrolase|glutaminase C|glutaminase, phosphate-activated
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Interactome
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Pathway
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Disease
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