GLO1 polyclonal antibody (A01)
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More Files
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length recombinant GLO1.
Immunogen
GLO1 (AAH11365, 1 a.a. ~ 184 a.a) full-length recombinant protein with GST tag.
Sequence
MAEPQPPSGGLTDEAALSYCSDADPSTKDFLLQQTMLRVKDPKKSLDFYTRVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKATLELTHNWGTEDDETQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKFVKKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM
Host
Mouse
Reactivity
Human
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (46.35 KDa) .
Storage Buffer
50 % glycerol
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of GLO1 expression in transfected 293T cell line by GLO1 polyclonal antibody (A01).
Lane1:GLO1 transfected lysate (Predicted MW: 20.7 KDa).
Lane2:Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
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Gene Info — GLO1
Entrez GeneID
2739GeneBank Accession#
BC011365Protein Accession#
AAH11365Gene Name
GLO1
Gene Alias
GLOD1, GLYI
Gene Description
glyoxalase I
Gene Ontology
HyperlinkGene Summary
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq
Other Designations
OTTHUMP00000016339|glyoxalase domain containing 1|lactoyl glutathione lyase|lactoylglutathione lyase
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Interactome
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Pathway
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Disease
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Publication Reference
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Glyoxalase I (GLO1) is up-regulated in pancreatic cancerous tissues compared with related non-cancerous tissues.
Wang Y, Kuramitsu Y, Ueno T, Suzuki N, Yoshino S, Iizuka N, Akada J, Kitagawa T, Oka M, Nakamura K.
Anticancer Research 2012 Aug; 32(8):3219.
Application:WB-Tr, Human, Human pancreatic cancer.
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Polyproline-rod approach to isolating protein targets of bioactive small molecules: isolation of a new target of indomethacin.
Sato S, Kwon Y, Kamisuki S, Srivastava N, Mao Q, Kawazoe Y, Uesugi M.
Journal of the American Chemical Society 2007 Jan; 129(4):873.
Application:WB, Human, A-549 cells.
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Glyoxalase I (GLO1) is up-regulated in pancreatic cancerous tissues compared with related non-cancerous tissues.
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