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Last updated: 2020/8/9
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GLI1 monoclonal antibody (M01J), clone 1F12 CX Grade

  • Catalog # : H00002735-M01J
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant GLI1.
  • Immunogen:
  • GLI1 (AAH13000, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • GPLPRAPSISTVEPKREREGGPIREESRLTVPEGAMKPQPSPGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTGLSTLRRLEN
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2a Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Interspecies Antigen Sequence:
  • Mouse (88%); Rat (89%)
  • Applications
  • Immunofluorescence
  • Immunofluorescence enlargeenlarge this image
  • Immunofluorescence of monoclonal antibody to GLI1 on HeLa cell . [antibody concentration 10 ug/ml]
  • ELISA
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • Proximity Ligation Analysis of protein-protein interactions between SUFU and GLI1. HeLa cells were stained with anti-SUFU rabbit purified polyclonal 1:1200 and anti-GLI1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
  • Application Image
  • ELISA
  • In situ Proximity Ligation Assay (Cell)
  • <em>In situ</em> Proximity Ligation Assay (Cell)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 2735
  • Gene Name:
  • GLI1
  • Gene Alias:
  • GLI
  • Gene Description:
  • glioma-associated oncogene homolog 1 (zinc finger protein)
  • Gene Summary:
  • This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • glioma-associated oncogene homolog (zinc finger protein),glioma-associated oncogene homolog 1
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