GLE1L 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GLE1 transfected lysate.
Western Blot
Lane 1: GLE1 transfected lysate ( 79.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GLE1L full-length
Host
Human
Theoretical MW (kDa)
79.9
Interspecies Antigen Sequence
Mouse (82); Rat (83)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GLE1L antibody (H00002733-B01P) by Western Blots.
SDS-PAGE Gel
GLE1 transfected lysate.
Western Blot
Lane 1: GLE1 transfected lysate ( 79.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GLE1
Entrez GeneID
2733GeneBank Accession#
BC030012Protein Accession#
AAH30012Gene Name
GLE1
Gene Alias
GLE1L, LCCS, LCCS1, hGLE1
Gene Description
GLE1 RNA export mediator homolog (yeast)
Omim ID
603371Gene Ontology
HyperlinkGene Summary
This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
GLE1 RNA export mediator homolog|GLE1 RNA export mediator-like|GLE1-like, RNA export mediator|OTTHUMP00000022280|OTTHUMP00000022281
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Interactome
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Disease
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