GLA 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GLA transfected lysate.
Western Blot
Lane 1: GLA transfected lysate ( 48.8 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GLA full-length
Host
Human
Theoretical MW (kDa)
48.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GLAantibody (H00002717-B01)byWesternBlots.
SDS-PAGE Gel
GLA transfected lysate.
Western Blot
Lane 1: GLA transfected lysate ( 48.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GLA
Entrez GeneID
2717GeneBank Accession#
NM_000169.1Protein Accession#
NP_000160.1Gene Name
GLA
Gene Alias
GALA
Gene Description
galactosidase, alpha
Gene Ontology
HyperlinkGene Summary
This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties. [provided by RefSeq
Other Designations
OTTHUMP00000023679|agalsidase alfa|alpha-D-galactosidase A|alpha-D-galactoside galactohydrolase 1|alpha-gal A|alpha-galactosidase A|melibiase
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Interactome
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Pathway
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Disease
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