GK 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GK transfected lysate.
Western Blot
Lane 1: GK transfected lysate ( 57.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GK full-length
Host
Human
Theoretical MW (kDa)
57.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GK antibody (H00002710-B01) by Western Blots.
SDS-PAGE Gel
GK transfected lysate.
Western Blot
Lane 1: GK transfected lysate ( 57.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GK
Entrez GeneID
2710GeneBank Accession#
NM_000167.3Protein Accession#
-Gene Name
GK
Gene Alias
GK1, GKD
Gene Description
glycerol kinase
Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the FGGY kinase family of proteins and encodes glycerol kinase. Glycerol kinase is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Defects in this gene are the cause of glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
ATP:glycerol 3-phosphotransferase|glycerokinase
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Interactome
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