GJB3 monoclonal antibody (M01), clone 3B4-1B3
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant GJB3.
Immunogen
GJB3 (AAH12918.1, 1 a.a. ~ 270 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDWKTLQALLSGVNKYSTAFGRIWLSVVFVFRVLVYVVAAERVWGDEQKDFDCNTKQPGCTNVCYDNYFPISNIRLWALQLIFVTCPSLLVILHVAYREERERRHRQKHGDQCAKLYDNAGKKHGGLWWTYLFSLIFKLIIEFLFLYLLHTLWHGFNMPRLVQCANVAPCPNIVDCYIARPTEKKIFTYFMVGASAVCIVLTICELCYLICHRVLRGLHKDKPRGGCSPSSSASRASTCRCHHKLVEAGEVDPDPGNNKLQASAPNLTPI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (83); Rat (83)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (55.44 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot (Cell lysate)
GJB3 monoclonal antibody (M01), clone 3B4-1B3 Western Blot analysis of GJB3 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of GJB3 expression in transfected 293T cell line by GJB3 monoclonal antibody (M01), clone 3B4-1B3.
Lane 1: GJB3 transfected lysate(31 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GJB3 is approximately 0.3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to GJB3 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — GJB3
Entrez GeneID
2707GeneBank Accession#
BC012918Protein Accession#
AAH12918.1Gene Name
GJB3
Gene Alias
CX31, DFNA2, EKV, FLJ22486, MGC102938
Gene Description
gap junction protein, beta 3, 31kDa
Gene Ontology
HyperlinkGene Summary
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq
Other Designations
OTTHUMP00000004183|OTTHUMP00000004184|connexin 31
-
Interactome
-
Disease
-
Publication Reference
-
EKV mutant connexin 31 associated cell death is mediated by ER stress.
Tattersall D, Scott CA, Gray C, Zicha D, Kelsell DP.
Human Molecular Genetics 2009 Sep; 18(24):4734.
Application:IF, WB-Ce, Human, HeLa cells.
-
EKV mutant connexin 31 associated cell death is mediated by ER stress.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com