GIF 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
GIF transfected lysate.
Western Blot
Lane 1: GIF transfected lysate ( 45.4 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GIF full-length
Host
Human
Theoretical MW (kDa)
45.4
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GIF antibody (H00002694-B01) by Western Blots.
SDS-PAGE Gel
GIF transfected lysate.
Western Blot
Lane 1: GIF transfected lysate ( 45.4 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GIF
Entrez GeneID
2694GeneBank Accession#
NM_005142.2Protein Accession#
-Gene Name
GIF
Gene Alias
IF, IFMH, INF, TCN3
Gene Description
gastric intrinsic factor (vitamin B synthesis)
Gene Ontology
HyperlinkGene Summary
This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq
Other Designations
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Interactome
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Disease
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