GIF monoclonal antibody (M03), clone 1D9
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More Files
- More Functions
Immunoprecipitation
Immunoprecipitation of GIF transfected lysate using anti-GIF monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GIF MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GIF is approximately 0.3ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to GIF on HeLa cell . [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant GIF.
Immunogen
GIF (NP_005133.2, 318 a.a. ~ 417 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
INNQLRGVELLFNETINVSVKSGSVLLVVLEEAQRKNPMFKFETTMTSWGLVVSSINNIAENVNHKTYWQFLSGVTPLNEGVADYIPFNHEHITANFTQY
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Immunoprecipitation
Immunoprecipitation of GIF transfected lysate using anti-GIF monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GIF MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GIF is approximately 0.3ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to GIF on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — GIF
Entrez GeneID
2694GeneBank Accession#
NM_005142Protein Accession#
NP_005133.2Gene Name
GIF
Gene Alias
IF, IFMH, INF, TCN3
Gene Description
gastric intrinsic factor (vitamin B synthesis)
Gene Ontology
HyperlinkGene Summary
This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq
Other Designations
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Interactome
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Disease
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