GHRH 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GHRH full-length
Host
Human
Theoretical MW (kDa)
11.99
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GHRH antibody (H00002691-B01) by Western Blots.
Western Blot
Lane 1: GHRH transfected lysate ( 12.4 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
GHRH transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GHRH
Entrez GeneID
2691GeneBank Accession#
NM_021081Protein Accession#
NP_066567Gene Name
GHRH
Gene Alias
GHRF, GRF, MGC119781
Gene Description
growth hormone releasing hormone
Omim ID
139190Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the glucagon family and is a preproprotein that is produced in the hypothalamus. The preproprotein is cleaved to form a 44 aa factor, also called somatocrinin, that acts to stimulate growth hormone release from the pituitary. Variant receptors for somatocrinin have been found in several types of tumors, and antagonists of these receptors can inhibit the growth of the tumors. Defects in this gene are a cause of dwarfism, while hypersecretion of the encoded protein is a cause of gigantism. [provided by RefSeq
Other Designations
OTTHUMP00000030915|growth hormone releasing factor|sermorelin|somatocrinin|somatoliberin
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