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Last updated: 2016/10/23
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GCK 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00002645-T02
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-GCK full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 51.26
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-GCK antibody (H00002645-B02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00002645-T02
    GCK transfected lysate.
    Western Blot
    QC Testing of H00002645-T02
    Lane 1: GCK transfected lysate ( 51.26 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 2645
  • Gene Name:
  • GCK
  • Gene Alias:
  • Gene Description:
  • glucokinase (hexokinase 4)
  • Gene Summary:
  • Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq
  • Other Designations:
  • ATP:D-hexose 6-phosphotransferase,OTTHUMP00000024521,OTTHUMP00000159308,glucokinase,hexokinase D, pancreatic isozyme
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