GCK 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GCK full-length
Host
Human
Theoretical MW (kDa)
52.2
Interspecies Antigen Sequence
Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GCK antibody (H00002645-B01) by Western Blots.
SDS-PAGE Gel
GCK transfected lysate
Western Blot
Lane 1: GCK transfected lysate ( 52.2 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GCK
Entrez GeneID
2645GeneBank Accession#
NM_000162Protein Accession#
NP_000153Gene Name
GCK
Gene Alias
GK, GLK, HHF3, HK4, HKIV, HXKP, MODY2
Gene Description
glucokinase (hexokinase 4)
Gene Ontology
HyperlinkGene Summary
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq
Other Designations
ATP:D-hexose 6-phosphotransferase|OTTHUMP00000024521|OTTHUMP00000159308|glucokinase|hexokinase D, pancreatic isozyme
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Interactome
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