This antibody pair set comes with matched antibody pair to detect and quantify protein level of human GCK.
Quality Control Testing:
Standard curve using GCK 293T overexpression lysate (non-denatured) as an analyte.
Sandwich ELISA detection sensitivity ranging from approximately 729x to 3x dilution of the GCK 293T overexpression lysate (non-denatured).
Antibody pair set content: 1. Capture antibody: mouse monoclonal anti-GCK, IgG1 Kappa (100 ug) 2. Detection antibody: rabbit purified polyclonal anti-GCK (50 ug) *Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq