GCG 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GCG transfected lysate.
Western Blot
Lane 1: GCG transfected lysate ( 20.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GCG full-length
Host
Human
Theoretical MW (kDa)
20.9
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GCG antibody (H00002641-D01P) by Western Blots.
SDS-PAGE Gel
GCG transfected lysate.
Western Blot
Lane 1: GCG transfected lysate ( 20.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GCG
Entrez GeneID
2641GeneBank Accession#
NM_002054Protein Accession#
NP_002045.1Gene Name
GCG
Gene Alias
GLP1, GLP2, GRPP
Gene Description
glucagon
Omim ID
138030Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq
Other Designations
glicentin-related polypeptide|glucagon-like peptide 1|glucagon-like peptide 2
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Interactome
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