GBE1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GBE1 transfected lysate.
Western Blot
Lane 1: GBE1 transfected lysate ( 77.33 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GBE1 full-length
Host
Human
Theoretical MW (kDa)
77.33
Interspecies Antigen Sequence
Mouse (92); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GBE1 antibody (H00002632-B01) by Western Blots.
SDS-PAGE Gel
GBE1 transfected lysate.
Western Blot
Lane 1: GBE1 transfected lysate ( 77.33 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GBE1
Entrez GeneID
2632GeneBank Accession#
BC012098.1Protein Accession#
AAH12098.1Gene Name
GBE1
Gene Alias
GBE
Gene Description
glucan (1,4-alpha-), branching enzyme 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease). [provided by RefSeq
Other Designations
amylo-(1,4 to 1,6) transglucosidase|amylo-(1,4 to 1,6) transglycosylase|glycogen branching enzyme|glycogen storage disease type IV
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Interactome
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Pathway
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Disease
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