GARS 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GARS transfected lysate.
Western Blot
Lane 1: GARS transfected lysate ( 75.46 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GARS full-length
Host
Human
Theoretical MW (kDa)
75.46
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GARS antibody (H00002617-B01) by Western Blots.
SDS-PAGE Gel
GARS transfected lysate.
Western Blot
Lane 1: GARS transfected lysate ( 75.46 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GARS
Entrez GeneID
2617GeneBank Accession#
NM_002047.1Protein Accession#
ENSP00000373918Gene Name
GARS
Gene Alias
CMT2D, DSMAV, GlyRS, HMN5, SMAD1
Gene Description
glycyl-tRNA synthetase
Gene Ontology
HyperlinkGene Summary
This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. [provided by RefSeq
Other Designations
Charcot-Marie-Tooth neuropathy 2D|Charcot-Marie-Tooth neuropathy, neuronal type, D|glycine tRNA ligase
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Interactome
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Pathway
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Disease
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