GAMT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GAMT full-length
Host
Human
Theoretical MW (kDa)
26.07
Interspecies Antigen Sequence
Mouse (88); Rat (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GAMT antibody (H00002593-B01) by Western Blots.
SDS-PAGE Gel
GAMT transfected lysate.
Western Blot
Lane 1: GAMT transfected lysate ( 26.07 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GAMT
Entrez GeneID
2593GeneBank Accession#
NM_000156.4Protein Accession#
NP_000147.1Gene Name
GAMT
Gene Alias
PIG2, TP53I2
Gene Description
guanidinoacetate N-methyltransferase
Omim ID
601240Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a methyltransferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl donor. Defects in this gene have been implicated in neurologic syndromes and muscular hypotonia, probably due to creatine deficiency and accumulation of guanidinoacetate in the brain of affected individuals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq
Other Designations
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