GALT monoclonal antibody (M01), clone 4C11
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant GALT.
Immunogen
GALT (AAH15045, 1 a.a. ~ 379 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSRSGTDPQQRQQASEADAAAATFRANDHQHIRYNPLQDEWVLVSAHRMKRPWQGQVEPQLLKTVPRHDPLNPLCPGAIRANGEVNPQYDSTFLFDNDFPALQPDAPSPGPSDHPLFQAKSARGVCKVMCFHPWSDVTLPLMSVPEIRAVVDAWASVTEELGAQYPWVQIFENKGAMMGCSNPHPHCQVWASSFLPDIAQREERSQQAYKSQHGEPLLMEYSRQELLRKERLVLTSEHWLVLVPFWATWPYQTLLLPRRHVRRLPELTPAERDDLASIMKKLLTKYDNLFETSFPYSMGWHGAPTGSEAGANWDHWQLHAHYYPPLLRSATVRKFMVGYEMLAQAQRDLTPEQAAERLRALPEVHYHLGQKDRETATIA
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (92); Rat (89)
Isotype
IgG2a kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (67.43 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
GALT monoclonal antibody (M01), clone 4C11 Western Blot analysis of GALT expression in K-562 ( Cat # L009V1 ).Western Blot (Transfected lysate)
Western Blot analysis of GALT expression in transfected 293T cell line by GALT monoclonal antibody (M01), clone 4C11.
Lane 1: GALT transfected lysate(43 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to GALT on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 1 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GALT is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — GALT
Entrez GeneID
2592GeneBank Accession#
BC015045Protein Accession#
AAH15045Gene Name
GALT
Gene Alias
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Gene Description
galactose-1-phosphate uridylyltransferase
Gene Ontology
HyperlinkGene Summary
Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. [provided by RefSeq
Other Designations
OTTHUMP00000021298|galactose-1-phosphate uridyl transferase
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Interactome
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Pathway
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Disease
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