GALNT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GALNT1 transfected lysate.
Western Blot
Lane 1: GALNT1 transfected lysate ( 11.66 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GALNT1 full-length
Host
Human
Theoretical MW (kDa)
11.66
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GALNT1 antibody (H00002589-B01) by Western Blots.
SDS-PAGE Gel
GALNT1 transfected lysate.
Western Blot
Lane 1: GALNT1 transfected lysate ( 11.66 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GALNT1
Entrez GeneID
2589GeneBank Accession#
BC047746.1Protein Accession#
AAH47746.1Gene Name
GALNT1
Gene Alias
GALNAC-T1
Gene Description
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1)
Omim ID
602273Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. Transcript variants derived from this gene that utilize alternative polyA signals have been described in the literature. [provided by RefSeq
Other Designations
GalNAc transferase 1|UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1|polypeptide N-acetylgalactosaminyltransferase 1|protein-UDP acetylgalactosaminyltransferase 1
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Interactome
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Pathway
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Disease
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