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GALE 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00002582-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-GALE full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 38.3
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-GALE antibody (H00002582-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00002582-T01
    GALE transfected lysate.
    Western Blot
    QC Testing of H00002582-T01
    Lane 1: GALE transfected lysate ( 38.3 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 2582
  • Protein Accession#:
  • -
  • Gene Name:
  • GALE
  • Gene Alias:
  • FLJ95174,FLJ97302,SDR1E1
  • Gene Description:
  • UDP-galactose-4-epimerase
  • Gene Summary:
  • This gene encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form). Multiple alternatively spliced transcripts encoding the same protein have been identified. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000002991,OTTHUMP00000044857,UDP galactose-4'-epimerase,galactose-4-epimerase, UDP-,galactowaldenase,short chain dehydrogenase/reductase family 1E, member 1
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