GALC 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GALC transfected lysate.
Western Blot
Lane 1: GALC transfected lysate ( 75.10 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GALC full-length
Host
Human
Theoretical MW (kDa)
75.1
Interspecies Antigen Sequence
Mouse (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GALC antibody (H00002581-D01P) by Western Blots.
SDS-PAGE Gel
GALC transfected lysate.
Western Blot
Lane 1: GALC transfected lysate ( 75.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GALC
Entrez GeneID
2581GeneBank Accession#
BC036518.2Protein Accession#
AAH36518.1Gene Name
GALC
Gene Alias
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Gene Description
galactosylceramidase
Gene Ontology
HyperlinkGene Summary
This gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease, also known as globoid cell leukodystrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
GALCERase|OTTHUMP00000027906|galactocerebrosidase|galactocerebroside beta-galactosidase|galactosylceramide beta-galactosidase|galactosylceraminidase
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Interactome
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Pathway
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