GAK 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GAK transfected lysate.
Western Blot
Lane 1: GAK transfected lysate ( 45.87 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GAK full-length
Host
Human
Theoretical MW (kDa)
45.87
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GAK antibody (H00002580-B01) by Western Blots.
SDS-PAGE Gel
GAK transfected lysate.
Western Blot
Lane 1: GAK transfected lysate ( 45.87 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GAK
Entrez GeneID
2580GeneBank Accession#
BC008668.1Protein Accession#
AAH08668.1Gene Name
GAK
Gene Alias
FLJ16629, FLJ40395, MGC99654
Gene Description
cyclin G associated kinase
Omim ID
602052Gene Ontology
HyperlinkGene Summary
In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. [provided by RefSeq
Other Designations
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Interactome
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Disease
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