XRCC6 monoclonal antibody (M01), clone 4C2-1A6
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More Files
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Western Blot (Cell lysate)
XRCC6 monoclonal antibody (M01), clone 4C2-1A6 Western Blot analysis of XRCC6 expression in C32 ( Cat # L002V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of XRCC6 expression in transfected 293T cell line by G22P1 monoclonal antibody (M01), clone 4C2-1A6.
Lane 1: XRCC6 transfected lysate(70 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to XRCC6 on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged XRCC6 is approximately 0.1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to XRCC6 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (92.73 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant XRCC6.
Immunogen
XRCC6 (AAH08343.1, 1 a.a. ~ 609 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFDMSIQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWVCANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGGFDISLFYRDIISIAEDEDLRVHFEESSKLEDLLRKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (83); Rat (83)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (92.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
XRCC6 monoclonal antibody (M01), clone 4C2-1A6 Western Blot analysis of XRCC6 expression in C32 ( Cat # L002V1 ).Western Blot (Transfected lysate)
Western Blot analysis of XRCC6 expression in transfected 293T cell line by G22P1 monoclonal antibody (M01), clone 4C2-1A6.
Lane 1: XRCC6 transfected lysate(70 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to XRCC6 on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged XRCC6 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to XRCC6 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — XRCC6
Entrez GeneID
2547GeneBank Accession#
BC008343Protein Accession#
AAH08343.1Gene Name
XRCC6
Gene Alias
CTC75, CTCBF, G22P1, KU70, ML8, TLAA
Gene Description
X-ray repair complementing defective repair in Chinese hamster cells 6
Omim ID
152690Gene Ontology
HyperlinkGene Summary
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq
Other Designations
ATP-dependent DNA helicase II, 70 kDa subunit|CTC box binding factor 75 kDa subunit|Ku autoantigen p70 subunit|Ku autoantigen, 70kDa|OTTHUMP00000028581|thyroid autoantigen 70kD (Ku antigen)|thyroid autoantigen 70kDa (Ku antigen)|thyroid-lupus autoantigen
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Interactome
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Pathway
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Disease
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Publication Reference
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Lysine-specific demethylase 2A enhances binding of various nuclear factors to CpG-rich genomic DNAs by action of its CXXC-PHD domain.
Iuchi S, Paulo JA.
Scientific Reports 2019 Apr; 9(1):5496.
Application：PI, WB-Re, Recombinant proteins.
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Mesenchymal subtype of glioblastomas with high DNA-PKcs expression is associated with better response to radiotherapy and temozolomide.
Pinel B, Duchesne M, Godet J, Milin S, Berger A, Wager M, Karayan-Tapon L.
Journal of Neuro-Oncology 2017 Apr; 132(2):287.
Application：IHC, Human, Patients with Glioblastoma multiforme (GBM).
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DNA-PKcs expression predicts response to radiotherapy in prostate cancer.
Bouchaert P, Guerif S, Debiais C, Irani J, Fromont G.
International Journal of Radiation Oncology, Biology, Physics 2012 Dec; 84(5):1179.
Application：IHC-P, Human, Prostate cancer.
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Myonuclear breakdown in sporadic inclusion body myositis is accompanied by DNA double strand breaks.
Nishii M, Nakano S, Nakamura S, Wate R, Shinde A, Kaneko S, Kusaka H.
Neuromuscular Disorders 2011 May; 21(5):345.
Application：IHC, Human, Muscle.
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Lysine-specific demethylase 2A enhances binding of various nuclear factors to CpG-rich genomic DNAs by action of its CXXC-PHD domain.
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