GAGE1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GAGE1 transfected lysate.
Western Blot
Lane 1: GAGE1 transfected lysate ( 15.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GAGE1 full-length
Host
Human
Theoretical MW (kDa)
15.6
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GAGE1 antibody (H00002543-B01) by Western Blots.
SDS-PAGE Gel
GAGE1 transfected lysate.
Western Blot
Lane 1: GAGE1 transfected lysate ( 15.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GAGE1
Entrez GeneID
2543GeneBank Accession#
NM_001468.3Protein Accession#
-Gene Name
GAGE1
Gene Alias
MGC33825
Gene Description
G antigen 1
Omim ID
300594Gene Ontology
HyperlinkGene Summary
This gene belongs to a family of genes that are expressed in a variety of tumors but not in normal tissues, except for the testis. The sequences of the family members are highly related but differ by scattered nucleotide substitutions. The GAGE1 cDNA contains a 143-bp insertion, located in the coding sequence near the termination codon, that is absent from the other cDNAs.The antigenic peptide YRPRPRRY, which is also encoded by several other family members, is recognized by autologous cytolytic T lymphocytes. Nothing is presently known about the function of this protein. An alternatively spliced transcript variant has been found for this gene. [provided by RefSeq
Other Designations
MZ2-F antigen|OTTHUMP00000025848
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