G6PD 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-G6PD full-length
Host
Human
Theoretical MW (kDa)
59.3
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-G6PD antibody (H00002539-D01P) by Western Blots.
SDS-PAGE Gel
G6PD transfected lysate.
Western Blot
Lane 1: G6PD transfected lysate ( 59.30 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — G6PD
Entrez GeneID
2539GeneBank Accession#
NM_001042351.1Protein Accession#
NP_001035810.1Gene Name
G6PD
Gene Alias
G6PD1
Gene Description
glucose-6-phosphate dehydrogenase
Omim ID
305900Gene Ontology
HyperlinkGene Summary
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000026034|glucose-6-phosphate 1-dehydrogenase|glucose-6-phosphate dehydrogenase, G6PD
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