FUT3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-FUT3 full-length
Host
Human
Theoretical MW (kDa)
39.82
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FUT3 antibody (H00002525-B01) by Western Blots.
SDS-PAGE Gel
FUT3 transfected lysate.
Western Blot
Lane 1: FUT3 transfected lysate ( 42.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — FUT3
Entrez GeneID
2525GeneBank Accession#
NM_000149.1Protein Accession#
NP_000140.1Gene Name
FUT3
Gene Alias
CD174, FT3B, FucT-III, LE, Les, MGC131739
Gene Description
fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
Omim ID
111100Gene Ontology
HyperlinkGene Summary
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq
Other Designations
Lewis FT|alpha-(1,3/1,4)-fucosyltransferase|blood group Lewis alpha-4-fucosyltransferase|fucosyltransferase 3|galactoside 3(4)-L-fucosyltransferase
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Interactome
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